grafting frame holder

Suits wood and plastic frames. We focus on beekeeping equipment, tools, queen bees, colonies (swarms) and much more. Standard Frame Holders that Suits wood & plastic frames available by Beequip. Adapter trimming was applied to all small RNA sequencing reads of tissue and plasma samples, followed by mapping to the GRCh38 reference genome with Bowtie (v1.2.2). P.P. K.D.P. Photos: Kate Anton, Penn State. Yu YP, et al. The frame perch fits snugly over the edge of the hive body to conveniently and securely hold your frames during a routine inspection. Along with a mentor and some educational reading, these grafting tools will help get your queen rearing project off the ground. In this study, we generated a comprehensive dataset that allows exploration of the complex transcriptome landscape of EAC and precursor lesions (HGD, NDB) in 17 patients. Technical validation of the data. The upregulation of hsa-miR-194 and SHH, and downregulation of SUFU compared to healthy tissue is also observed in our NDB tissue data as well as in the EAC and HGD tissue samples (Figs. MicroRNA-145 in Barretts oesophagus: Regulating BMP4 signalling via GATA6. Results obtained from these analyses are provided in Supplementary Table5. The remaining sequence was aligned against the human reference genome build GRCh38 using STAR (v2.6.0c, two-step mode). Pools were concentrated with ethanol precipitation and quantification with the Qubit 2.0 fluorometer (Thermo Fisher Scientific). Description Completely assembled 9" Deep frame designed to hold two JZBZ (JZ-BZ) Plastic Cell Bars or two Wood Cell Bars. We offer quality beekeeping tools & equipment New Zealand wide. Custom items not shown in this catalog along with items marked with "Does not qualify for free shipping" are excluded from the free shipping offer. The R packages edgeR (v3.28.1) and limma (v3.42.2) were used for normalization (Trimmed Mean of M-values) differential gene expression (tissue)/ abundance (plasma) analysis, respectively. Libraries were prepared with the TruSeq Stranded mRNA Library Prep kit (Illumina), using 100ng of RNA as input material. Description. The clever design gives you quick and easy access to your cards . 1Translational Oncogenomics and Bioinformatics Lab, Cancer Research Institute Ghent (CRIG), Ghent, Belgium, 2OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent, Belgium, 3 Department of Biomolecular Medicine, Ghent University, Ghent, Belgium, 4Department of Gastro-Intestinal Surgery, University Hospital Ghent, Belgium, 5Department of Oncogenomics, Academic Medical Center (AMC), Amsterdam, The Netherlands, 6Department of Gastroenterology and Hepatology, University Hospital Ghent, Ghent, Belgium, 7Department of Human Structure and Repair, Ghent University, Ghent, Belgium. This article is part of a series on biology and techniques for queen rearing from the Center for Pollinator Research at The Pennsylvania State University. K.S. The main risk factors to develop EAC are gastro-esophageal reflux disease (GERD), Barretts esophagus, smoking and age above 50 years5. Based on the polyA+ sequencing data (tissue) and mRNA capture sequencing data (plasma), mutation analysis was performed. To graft, lower the grafting tool behind the curve of the larva, maneuver the tool under the larva and the small pool of royal jelly, and gently lift and transfer the larva to the center of the cell cup (Figure 6). If you don't follow our itemsreturn policy, you may not receive a full refund. Despite improved treatment strategies, the five-year survival rate remains unacceptably low (1025%)3,4. While the adjusted p-values (Benjamini-Hochberg) are not significant in this dataset, a heatmap of the top ten most abundant circRNAs (p-values are below 2.36103) comparing EAC with NDB samples shows that plasma circRNAs may have biomarker potential, but needs further validation (Fig. A spring-loaded retractable mechanism allows the larva and royal jelly to be moved from the tip into the cell cup. Wang S, et al. Delivered straight to your inbox(you can unsubscribe anytime), 2023 BuzzbeeOwned by Buzzbee International, Wax Foundation Frame Assembly and Embedding Kits. Reads were trimmed with Cutadapt (v.1.18), low quality reads were removed with a custom script, retaining only reads where 80% of the read has a Phred quality score of at least 20 and duplicates were removed with Clumpify BBMap (v38.26). The relationship between miRNA and mRNA expression can thus be studied in our data. Cancer transcriptome profiling at the juncture of clinical translation. Pre-processing of mRNA sequencing data of plasma and tissue samples included 3-end trimming, adapter removal and filtering (discard reads smaller than 20 nt) using Cutadapt (v1.18). Double End Angled Stainless-Steel Grafting Tool for Queen Rearing. We want to ensure youre satisfied with your Buzzbee shopping experience. Blood samples were collected in a 6ml EDTA waste tube followed by a 9ml sodium citrate (3.2%) VACUETTE blood tube (Greiner Bio-One). Mechanisms that drive EAC development remain poorly understood. They are narrower than regular frames (facilitating movement in and out of the hive), and may be one of several designs. A damp towel draped over the frame will keep the humidity high and should be used to cover the part of the frame that is not in use. Before Esophageal cancer is the sixth most common cause of cancer-related death worldwide1. We accept returns of unused damaged or defective items within 30 days of delivery with receipt or proof of purchase. During a large graft, take grafted cells to the starter colonies a few at a time. These GSEA results (FDR<25%) are available in Supplementary Table3. Subscribe to get special offers, free giveaways, and once-in-a-lifetime deals. Follow-up time indicates time in months with the last known disease progression in brackets. De Preter K, Vandesompele J. Clemons NJ, Phillips WA, Lord RV. (sold separately) Complete Kit Includes Components to Produce 10 Queens: 1 Cell Grid 110 Brown Cell Cups 10 Hair Roller Cages You can contact us via * Phone* Email* Facebook Messenger* Contact us* Text message. Choose between Cell Bar Frame with 36 JZ's BZ's cell cups or Cell Bar Frame without cups. Suits all the usual Langstroth size frames sold in NZ. An experimental overview is shown in Fig. BEDTools (v2.26.0) was used to convert BAM files to fastq files. In our analyses, no significantly differentially expressed circRNAs were identified (Table7). I suggest making your own. In EAC samples, fewer unique circRNAs were found (353-1,165) compared to HGD (858-3,624) and NDB (1,237-3,683). Buzzbee Official shop for all thing beekeeping. 2d). HHS Vulnerability Disclosure, Help Athar A, et al. BLATThe BLAST-Like Alignment Tool. Revilla-Nuin B, et al. The circulating transcriptome as a source of cancer liquid biopsy biomarkers. Different contrasts were analyzed: comparing disease with healthy tissue (contrast 1), comparing disease tissue between groups (contrast 2), comparing disease versus healthy tissue samples of one group with the disease versus healthy tissue samples of another group (contrast 3), and comparing the three groups for the plasma samples (contrast 4). Deciphering the Unique MicroRNA Signature in Human Esophageal Adenocarcinoma. Neureiter D, et al. Queen Cell Production: Grafting and Graft-Free Methods, Skip to the beginning of the images gallery, The Hive and the Honey Bee by Lorenzo Langstroth, Queen Rearing Essentials by Lawrence John Connor. Using the sample clustering option in R2 for the plasma mRNA expression level data, a clear clustering of the samples according to sample identity, i.e. The beekeeper who only grafts occasionally will also appreciate this system. Specifically, GERD can cause progression from non-dysplastic Barretts esophagus (NDB) through the stages of low-grade dysplasia (LGD) to high-grade dysplasia (HGD), and eventually to invasive EAC6. Sample ID43_EAC was also excluded for downstream analyses, due to the lower library quality of the disease tissue sample. When hanging properly in the frame these cups should be facing downward. To a lesser extent, messenger RNA (mRNA) expression has been studied in EAC, HGD and NDB as well11,12. We use the bamboo grafting tool to graftlarvae into queen cups on the cell bar frame. 8600 Rockville Pike Subramanian A, et al. Better understanding of disease etiology along with the identification of novel prognostic and predictive biomarkers are urgently needed to improve the dismal survival probabilities. Wu X, et al. We cannot accept returns on hazardous materials. Tissue samples were obtained during endoscopy (NDB and HGD) or after surgical resection of the tumor (EAC). 2021. Plasma was prepared by centrifugation at 1,800g for 10min (full break and acceleration). Day 3: Eggs hatch. A capped queen cell and nurse bee. On the diagonal line are the number of reported genes in each gene set. Mastering this technique takes practice and repetition. The epidemic of oesophageal carcinoma: Where are we now? Cabibi D, et al. Identification of fusion genes provides valuable insights in the development of EAC and can potentially be used as biomarkers for detection or therapeutic targeting. Weaver JMJ, et al. The number of differentially expressed genes are depicted in Table7. Epidemiology, Diagnosis, and Management of Esophageal Adenocarcinoma. Upregulation of circulating mir130a is correlated with development of barretts esophagus and esophageal adenocarcinoma. Due to the ever-rising cost of outbound shipping, any order with backordered item(s) may be held until the backordered item(s) arrives. Whether your using a grafting tool or the Nicot system this frame works out great for keeping your unborn queens secure. This helped identify and filter out variants introduced by misaligned reads. J.V. Replace with the original frame. | AGP eGlass is part of AGP Group, one of the world's leading glazing manufacturers with a large portfolio of products for the automotive, marine, and security markets. Gene expression, Biomarkers, Oesophageal cancer, Data mining, Cancer genomics. This comprehensive dataset includes 17 patients with EAC, HGD or NDB. Results in this table are unfiltered, but in red are the fusion genes that have a high probability of being a false positive. 2a), reflecting the very good quality of the data. Tissue and plasma samples were single-end sequenced with a 75bp read length on a NextSeq 500 (Illumina) instrument according to the manufacturers instructions. Be the first to ask a question about this. All co-authors have critically reviewed the manuscript and approved the final version. We accept returns under the following conditions: We recommend you check your goods immediately upon receipt. After the desired number of grafts have been made, place the cell bars (with cell cups facing up) in the frame and transport it to the cell builder (Figure 7B). I know you're organized enough to use a relatively new comb to graft from, and thus can cut away the cell walls so that you're able to easily get your grafting tool under the larva you're aiming to pick up. Your payment information is processed securely. The past decade, profiling of nucleic acids isolated from liquid biopsies (e.g. Be the first to review Queen Rearing Grafting Frame. Ordering of mutations in preinvasive disease stages of esophageal carcinogenesis. Annotation was based on GRCh38, UCSC Genome Browser (reference genome) and GENCODE v20, Ensembl 84 (reference transcriptome). Deregulation of MYC is known to play a key role in the development of EAC36,37, indicating that tumor signal may be present in plasma. This technique allows beekeepers to create any number of queen cells that are easy to handle and transport. After 3 quality trimming, fusion genes were detected using a pipeline based on the FusionCatcher methodology (v0.99.7c). Along with a mentor and some educational reading, these grafting tools will help get your queen rearing project off the ground. The Garzini wallets are designed with an incredible attention to detail, timeless elegance and store your money in a surprising way. Fusion genes have been reported to be involved in cancer16, including EAC1719. The angle of the frame is more critical if your grafting old dark tough comb and need your light to see deeper into the cell. D.D.L. Epithelial mesenchymal transition and cancer stem cells in esophageal adenocarcinoma originating from barretts esophagus. Markers for epithelial mesenchymal transition (EMT) are of clinical relevance for a more targeted treatment38. Large scale queen cell production has allowed the commercial honey bee industry to meet both agricultural and hobbyist beekeeping demands.Convenient and inexpensive, these methods make it relatively easy to produce any number of queen cells. Day 4: Take out breeding frame to graft. Alternative graft-free methodsinclude the Jenter and Nicot systems, which allow for the production of numerous, moveable queen cells. If you do not do this, we will not be able to trace the goods to your account and may not be able to provide a refund or will reduce your refunds by the additional costs required to identify and trace the goods. Please enter your email address below to create account. For the purpose of this study, two collections of the Molecular Signatures Database (MSigDB) were used: the hallmark26 and the C2 chemical and genetic perturbations gene sets. Excellent quality, sturdy and well made! For grafting, the donor frame is often placed on an incline (Figure 3) and a flashlight or headlamp is used to identify the best larval candidates. Jun 7, 2016 - I saw a photo on the Internet last year and finally built myself of these Here's the prototype, just like the photo, but the top support interfered with my. contributed in the sample collection. While several differential miRNA abundance patterns in EAC or NDB plasma have been reported21,5358, there is only one overlapping miRNA (miR-194-5p) among these studies54,56. 3 William Street Tranmere SA 5073; 45 Gray Street Tranmere SA 5073; 36 Hectorville Road, Hectorville, SA 5073; 1 & 2/3 RODNEY AVENUE, TRANMERE Chen ZH, et al. Samples included in the boxplots are healthy and disease tissues from 3 patients with EAC, 5 with HGD and 7 with NDB. It shows a clustering of EAC samples versus HGD and NDB samples; (c) heatmap showing the relative expression of 35 overlapping differentially expressed genes (up and down) for tissue (left) and plasma (right) samples (Benjamini-Hochberg adjusted p-value<0.05); (d) the relative expression of top ten abundant circRNAs in plasma (EAC vs NDB) shown in a heatmap (p-value<2.36103); (e) boxplot representation of the relative expression of four of the most frequently reported up- and down regulated miRNAs (more than four times in literature) in EAC, HGD and/or NDB tissue samples compared to matched healthy esophageal tissue. Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles. Placing adequate shipping labels to Buzzbee to the address provided in step 1. by using both miRNA and mRNA data, as demonstrated by this example. Data reported in this study includes data for mRNA and miRNA expression, variant analysis, fusion gene detection and circRNAs (the latter only in plasma samples). Get a sheet of foundation and cut a couple of pieces to fit either side. Too much liquid, however, can drown the larvae. Range and mean (standard deviation) of unique protein coding genes (mRNAs), miRNAs and circRNAs found in tissue or plasma samples. AGP eGlass | 19.954 seguidores en LinkedIn. Exactly what I wanted. The frame perch fits snugly over the edge of the hive body to conveniently and securely hold your frames during a routine inspection. In stock, 83 units. Mapped reads were annotated to mature miRNAs as well as other small RNAs, including tRNA, rRNA, sn(o)RNAs and piRNAs. ExRNAQC Consortium. All code used for pre-processing mRNA and miRNA sequencing data is publicly available on GitHub (https://github.com/OncoRNALab/exRNAQC/blob/main/Preprocessing)61. Secrier M, et al. for it, in 2012. Odenthal M, et al. Grafting is a fun way to get more enjoyment from your home orchard. Oesophageal cancer survival in Europe: A EUROCARE-4 study. The raw sequencing data available at EGA were not part of the peer-reviewed content of this manuscript. Kent WJ. Grafting larvae to produce your own queens is a tricky process that becomes easier with practice. Required fields are marked *. You must arrange and pay for the items to be returned to us within 30 days of receipt. Regular price $5.50 German Queen Grafting Tool. This scale of queen production, along with the flexible behaviors inherent to honey bee colonies, are ultimately responsible for making honey bees the dominant pollinator in many landscapes. Despite high resolution endoscopy and virtual imaging techniques, detecting dysplasia in a long segment of Barretts esophagus remains challenging. Circulating miRNA profile in esophageal adenocarcinoma. Circulating miRNAs in patients with Barretts esophagus, high-grade dysplasia and esophageal adenocarcinoma. do you have a link where to get the microscope please? Accessibility Von Rahden BHA, Stein HJ, Phringer-Oppermann F, Sarbia M. c-myc amplification is frequent in esophageal adenocarcinoma and correlated with the upregulation of VEGF-A expression1. Fusion gene analysis was done on all tissue (polyA+ sequencing data) and plasma samples (mRNA capture sequencing data). Libraries were normalized based on qPCR quantification and pooled accordingly. Find all the essential tools for raising queen bees via grafting at Betterbee. At Buzzbee, we believe there is a better way to serve beekeepers. Wijnhoven BPL, et al. . Website Design by Stefanie Murrer. For example, comparing EAC tissue with healthy tissue the following relevant gene sets were significantly (FDR<1%) positively enriched in EAC: HALLMARK_EPITHELIAL_MESENCHYMAL_TRANSITION, HALLMARK_KRAS_SIGNALING_UP and WANG_ESOPHAGUS_CANCER_VS_NORMAL_UP35. You must return items in their original packaging and in the same condition as when you received them. We do not store credit card details nor have access to your credit card information. Figure 5. I also hold a mini Mag light in my non-grafting hand. Queen production allows beekeepers greater autonomy and independence, enabling individuals to better meet the goals of honey production, pollination, colony production, and genetic selection. document.getElementById( "ak_js_1" ).setAttribute( "value", ( new Date() ).getTime() ); 2018 Barnyard Bees. (A) Young larva in brood cell. Depristo MA, et al. A forum community dedicated to beekeeping, bee owners and enthusiasts. Items excluded from the offer are subject to change without notice. Stairs DB, et al. Verified Purchase. with a moist towel. The average number of reads for mRNA tissue and plasma samples throughout the pre-processing steps is shown in Table2. If you return items to us, you must follow instructions including. This modification makes it much easier, and faster. Frankell AM, et al. New frame holder for grafting | Grafting, Frame, Holder Apr 9, 2021 - I saw a photo on the Internet last year and finally built myself of these Here's the prototype, just like the photo, but the top support interfered with my. Cell Bar Frame with JZ's BZ's Cups - Langstroth Standard. Welded Stainless Steel construction for a tough long life. They are easy to make if you have any woodworking experience. The number of mapped reads remaining after the different pre-processing steps in tissue and plasma samples is shown in Table2. Cardoso J, et al. For each of the remaining variants, a BLAT (v3.5) analysis was performed to assess the quality of the reads contributing to the variant call31. The Fora platform includes forum software by XenForo. Differential MicroRNA signatures in the pathogenesis of barretts esophagus. Predictive value of microRNAs in the progression of Barrett esophagus to adenocarcinoma in a long-term follow-up study. (A) Plastic base mount cell cup. If you are unable to perform a graft, you will find these replacement bar frames very handy. Here, we demonstrate the potential of detecting fusion genes for EAC, HGD and NDB tissue and plasma samples. After using a grafting tool to remove larvae from brood cells, they are placed inside a queen cell cup which has been prefilled with royal jelly and is housed inside a cell bar frame. Afterwards, variants were filtered differently depending on the tissue of origin. Thrift AP. In tissue samples, potential fusion genes were identified in all samples. The pre-processed data is also uploaded in R2, allowing further exploration and visualization of the dataset. Trends in the incidence and survival of patients with esophageal cancer: A SEER database analysis. Ask us a question, when it's convenient for you. Expressed mRNAs, miRNAs and circRNAs have been identified in all tissue and/or plasma samples (Table4). Diverse alternative back-splicing and alternative splicing landscape of circular RNAs. However, as most small RNAs are typically around 2030 nucleotides in length, a good quality measure for the first 30 nucleotides of the 5-end of the read is more relevant in the context of small RNA expression analysis. Besides gene dysregulation, chromosomal rearrangements can result in fusion proteins. CYR61 and TAZ upregulation and focal epithelial to mesenchymal transition may be early predictors of barretts esophagus malignant progression. We used to place frames on the grass near the entry but it squashes some bees and there is a high risk that if the queen is there, she will crawl off the frame and could get lost. Stainless Steel Frame Holder Grafting Support For Bee Breeding Honeycomb Shelf For Bee Transfferring Product Specifications Details Images Company Profile We are a manufacturer and supplier of beekeeping equipment and bee products from China . Using the RNA sequencing data from tissue and plasma samples, variants were identified using the following pipeline (based on Piskol et al.29): the first ten bases of all paired-end reads of each sample were trimmed due to possible false positives that can occur here as a result of random priming. Blum AE, et al. A Quick Reference Guide to Honey Bee Parasites, Pests, Predators, and Diseases, A Field Guide to Honey Bees and Their Maladies, Mtodos para el control de Varroa destructor: un enfoque de manejo integrado de plagas. This was likely due to the low concentration (16.3ng/l) and low RNA quality score (3.4) (Supplementary Table2). Data requests can be made by contacting the Data Access Committee, as stated on the EGA information page of the study (https://ega-archive.org/studies/EGAS00001004939). 2b) is observed. MicroRNA expression in squamous cell carcinoma and adenocarcinoma of the esophagus: Associations with survival. This system allows you to raise up to 110 queens at one time. If we look into more detail we observe that some of the differentially expressed mRNAs in tissue of patients with EAC compared to NDB are also differentially abundant in the plasma samples (in the same direction). the contents by NLM or the National Institutes of Health. These authors jointly supervised this work: Jo Vandesompele, Piet Pattyn, Katleen De Preter. After the breeder queen lays new larvae into the comb box/cell cups, cell holders are attached to the cell cups. Differential gene expression and abundance analyses were performed for mRNAs, miRNAs and circRNAs in tissue and plasma. Shop Online now! Cielik M, Chinnaiyan AM. For all plasma samples, RNA was isolated from 200l plasma using the miRNeasy Serum/Plasma Kit (Qiagen) according to the manufacturers instructions. For Warranty enquiries, please view the Terms and Conditionsof Sale below orcontact usdirectly atsales@buzzbee.com.au. Reviewed in the United States on April 20, 2021. Low quality read pairs were removed using Biopython (v1.72) by keeping pairs with minimal 80% of their length having a Phred score greater or equal than 19. 2e and and3).3). In total, this comprehensive dataset consists of 102 sequenced libraries from 51 samples. Item Code: QR8. As input for the analysis, a ranked list based on log2 fold change of all genes was used. Libraries were normalized and samples were pooled accordingly. For sample ID37_NDB, reads from only one run have been included, since the first run contained an insufficient number of reads (less than 2,000) for this sample. As expected, fewer unique mRNAs and miRNAs were found in plasma compared to tissue samples. Will mount on the edge of a super up to 23mm thick. BEEMAN. It includes polyA+ RNA (tissue samples), mRNA capture-based (plasma) and miRNA expression profiling (tissue and plasma). I also see that you indicated your model is one with a 3 diopter lens, and they also sell one with a 5 diopter lens. For instance, microRNAs (miRNAs) have been identified in tissue biopsies as potential biomarkers for EAC, HGD and NDB10. Completely assembled 9 Deep frame designed to hold two JZBZ (JZ-BZ) Plastic Cell Bars or two Wood Cell Bars. Videos Help others learn more about this product by uploading a video! 10 Frame to 9 Frame Spacing Tool, Stainless, This frame perch is Beefy and very well made will stand up to heavy use A+. 4. Photos: Kate Anton, Penn State. Our cell bar frames hold three bars of queen cell cups for a total of 36 cups per frame. Whether your using a grafting tool or the Nicot system this frame works out great for keeping your unborn queens secure. Karczewski KJ, et al. First, reads are aligned onto the genome and transcriptome using TopHat2 in order to reduce false positive reads aligned in the TopHat-Fusion alignment. In most cases a restocking fee of 25% will be charged. Graft into cell cups. Not only will you love it, but your bees will love it too! Prior to these analyses, genes were filtered based on more than four counts in at least half of the samples per group (EAC, HGD, NDB). Clumpify (BBMap v38.26) was used for read duplicate removal for plasma samples only, due to the low RNA input. The mean sequencing quality per base (raw data) of the small RNA sequencing data (tissue and plasma) is higher than 28 for the first 60bp in all samples (Fig. and transmitted securely. Tables5 and and66 show the overlap of differentially expressed genes (adjusted p-value<0.05) between EAC and healthy tissue from three large studies11,12,35 and our own dataset. Three variants are known tumor mutations according to the COSMIC database in prostate cancer (COSM5564582), cervix or biliary tract cancer (COSM5493837), or large intestine cancer (COSM5756079). You understand and agree that attempting to opt-out by any means other than texting the opt-out commands above is not a reasonable means of opting out. Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Moreover, different blood fractions, including serum21,5559, plasma54 and extracellular vesicles53 were studied. Analysis of tissue and circulating microRNA expression during metaplastic transformation of the esophagus. Quantification of circulating mRNAs as a biomarker are much more challenging, due to their low concentration and fragmentation status in the blood. In the past decades, the incidence of esophageal adenocarcinoma has increased dramatically in Western populations. Bus P, et al. I would say a must have for any beekeeper! Message frequency is recurring. Prior to the analyses, count tables were filtered to include RNAs with more than four counts in at least half of the samples per group. Figure 4. A later subframe . Since extra-cellular RNA from plasma is highly fragmented and typically below the detection limit, the RNA concentration or integrity was not estimated. Tympanoplasty is the surgery used to repair a ruptured eardrum either on its own or as part of a larger operation involving the middle ear.

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